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The Alien Species VRE provides users with data relative to 13500 species belonging to 500 different terrestrial, freshwater, lagoon and marine sites. The data include several taxonomic groups from cyanobacteria to fish, amphibians, reptiles and mammals, inhabiting different habitats and their surroundings. This dataset was checked for quality: data cleaning and validation for taxonomic reliability and taxonomic consistency using different zoological and botanical taxonomic index and database (PESI, WORMS, and Catalogue of Life), and a semi-automatic data cleaning, through the tools available on the LifeWatch Italy Data Portal.